Sunday, July 12, 2015

WBUT B.Sc Microbiology MICROBIAL BIOTECHNOLOGY Question Paper 2012

WBUT B.Sc Microbiology MICROBIAL BIOTECHNOLOGY Question Paper 2012
University:West Bengal University of Technology
Course: B.Sc
Subject :  MICROBIAL BIOTECHNOLOGY
Year of  Question Paper : 2012


CS/B.Sc(H), MICROBIO/SEM-4/MBT-404/2012

2012

MICROBIAL BIOTECHNOLOGY

Time Allotted : 3 Hours Full Marks : 70

The figures in the margin indicate full marks.

Candidates are required to give their answers in their own words

as far as practicable.

GROUP – A

( Multiple Choice Type Questions )

1. Choose the correct answers for any ten of the following :

[Marks 10 × 1 = 10]

i) The alga which can produce SCP is

a) Chlorella b) Spirulina

c) Both (a) and (b) d) none of these.

ii) The ideal temperature for biogas production is :

a) 40° C-50° C b) 37° C

c) 30° C-40° C d) 25° C-27° C.

iii) Antibiotics is considered as

a) primary metabolite b) secondary metabolite

c) amino acid d) hormone.

iv) Ethanol can be used as

a) red petrol b) green petrol

c) blue petrol d) brown petrol.

v) Acetic acid is produced by

a) Clostridium botulinum

b) Gluconobacter aceti

c) Lactobacillus acidophilus

d) none of these.vi) The bacteria which degrades toluene is

a) Acinetobacter calcoacetions

b) Achromobacter xylosoxidars

c) Pseudomonas fluorescence

d) Ochrobacterium sp.

vii) The micro-organism which is not an inhabitant of the

biofilm in trickling filter is

a) Alcaligenes b) Ulothrix

c) Spirogyra d) Flavobacterium.

viii) The device which has high BOD removal efficiency is

a) Trickling filter

b) Rotating biological contactor

c) Aerobic ponds

d) Anaerobic ponds.

ix) The source(s) for production of Bio-diesel is/are

a) Jatropa b) Soybean

c) Microalgae d) all of these.

x) The organism(s) responsible for bioleaching of metals

from their respective ores is/are

a) Acidothiobacillus ferroxidans

b) Ferroplasma sp

c) both (a) and (b)

d) Pseudomonas aeruginosa.

xi) Tetracycline was first isolated from

a) Streptomyces gresius

b) Streptomyces aureofaciens

c) Actinomycetes

d) Paecilomyces sp.

xii) Vermicomposting involves conversion of carbon richorganic compounds to

a) sulphur rich compounds

b) nitrogen rich compounds

c) hydrogen rich compounds

d) sulphide rich compounds.

GROUP – B

( Short Answer Type Questions )

Answer any three of the following. [Marks 3 × 5 = 15]

2. What is the biochemistry for microbial leaching of FeS2 ?

Differentiate tank bioleaching from heap bioleaching. [Marks 3 + 2]

3. Why is Mercury considered as a health hazard ?

4. Write in general the production procedure for Antibiotics.

5. How does Pseudomonas putida degrade oil spill ? Name the

plasmid which is responsible for oil spill degradation. [Marks 4 + 1]

6. How does Bacillus thuringiensis work ? What do you mean

by primary and secondary metabolites ? [Marks 3 + 2]

GROUP – C

( Long Answer Type Questions )

Answer any three of the following. [Marks 3 × 15 = 45]

7. What is biofuel ? Classify biofuel. What are the microorganisms

involved in optimum biogas production ? What is

biodiesel and what are the sources from where biodiesel can

be isolated ? Describe in brief the production procedure for

biodiesel production. How can you characterize biodiesel ?

[Marks 2 + 3 + 3 + 5 + 2]

8. Differentiate chemostat from turbidostat. What is a batch

culture ? Draw the growth curve for a typical batch culture.

Derive Monod's equation for continuous culture. Differentiate

batch from continuous culture. What is fed batch culture ?[Marks 3 + 2 + 3 + 4 + 2 + 1]

9. Describe the process of Orleans method for vinegar

production. What is the role of acidification in vinegar

production ? Why acidification is never recommended before

the completion of sugar fermentation ? What is the cause for

darkening of vinegar ? Name the genera for acetic acid

bacteria. Write the reaction for acetic acid production where

oxygen is required. [Marks 5 + 2 + 2 + 2 + 2 + 2]

10. Why is bacterial heavy metal resistance evolved ? What are

metallothionein ? What are the five mechanisms to evade

metal toxicity ? Why is nutrient removal necessary for

tertiary sewage treatment ? [Marks 4 + 2 + 5 + 4]

11. What role is played by amylase in textile industries ? Name

the organisms used for assay of Vitamin B12. How can you

use micro-organisms as pesticides ? Give an example. What

are its advantages over chemical pesticides ? Justify. What is

the purpose and the strategy of biological phosphorus

removal in tertiary treatment of water ? [Marks 2 + 2 + 3 + 4 + 4]

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Saturday, July 11, 2015

WBUT B.Sc Microbiology Microbial Biotechnology question paper of 2013

WBUT   B.Sc Microbiology Microbial Biotechnology question paper of 2013
University: West Bengal University
Course: B.sc
Subject : Microbial Biotechnology
Year of  Question Paper : 2013


CS/B.Sc (H)/Micro. Bio./SEM-4/MBT-404/2013

2013

MICROBIAL BIOTECHNOLOGY

Time Allotted : 3 Hours Full Marks : 70

The figures in the margin indicate full marks.

Candidates are required to give their answers in their own words

as far as practicable.

GROUP – A

( Multiple Choice Type Questions )

1. Choose the correct alternatives for any ten of the

following : [Marks 10 × 1 = 10]

i) The following compound is a secondary metabolite

a) amino acid b) vitamins

c) hormones d) steroids.

ii) The main component of biogas

a) oxygen b) nitrogen

c) carbon dioxide d) methane.

iii) The trickling filter is used in waste water treatment in

a) primary treatment b) secondary treatment

c) tertiary treatment d) preliminary treatment.

iv) The organism which produces parasporal crystal

a) Bacillus subtilis b) Bacillus cereus

c) Pseudomonous sp. d) Bacillus thuringiensis.

v) The organism having OCT plasmid

a) Streptococcus pneumoniae

b) Staphylococcus aureus

c) Bacilus subtilis

d) Pseudomonas putida.

vi) The molecular weight of active Bt toxin is

a) 200 KDa b) 50 KDa

c) 60 KDa d) 100 KDa.

vii) Which of the following expression represents Monod

Equatioin ?

a) µ = µmax b) µ = µmax S / Ks

c) µ = µmaxS / Ks + S d)µ = µmaxS + Ks/S .

viii) The type of bacteria involved in bio-mineralization of ore

is

a) Mesophilic b) Thermophilic

c) Acidophilic d) All of these.

ix) The bioreactor used for pigment synthesis

a) Air lift b) Photo bioreactor

c) Bubble column d) Stirred tank.

x) The catalyst used in biodiesel production

a) Methanol b) Ethanol

c) Sodium hydroxide d) Potassium hydroxide.

xi) The gene of TOL plasmid responsible for production of

xylene oxidase

a) XyIC b) XyIA

c) XyID d) XyIL.

xii) Antifoam used in the fermentor is

a) Mustard oil b) Silicon oil

c) Olive oil d) None of these.

GROUP – B

( Short Answer Type Questions )

Answer any three of the following. [Marks 3 × 5 = 15]

2. Derive the equation for generation time ( g )

from the equation of mean growth rate equation ( k ).

3. Write a short note on air-lift fermentor.

4. Give the method of production of penicillin.

5. Differentiate between primary and secondary metabolites and

also their modes of production.

6. Describe the role of microorganisms in the treatment of

agricultural waste water.

GROUP – C

( Long Answer Type Questions )

Answer any three of the following. [Marks 3 × 15 = 45]

7. Write short notes on the following : [Marks 3 × 5]

a) Bioremediation

b) Biosorption

c) Bioleaching.

8. Define biodiesel. State the chemistry involved in the

production of biodiesel and discuss the role of it as a biofuel

used in transport processes. [Marks 3 + 8 + 4]

9. Describe the process of Orleans method for vinegar

production. What is the role of acidifcation in vinegar

production ? Why acidification if never recommended before

the completion of sugar fermentation ? What is the cause for

darkening of vinegar ? Name the genera for acetic acid

bacteria. Write the reaction for acetic acid production where

oxygen is required. [Marks 5 + 2 + 2 + 2 + 2 + 2]

10. Define chemostat and turbidostat. Compare between batch,

fedbatch and continuous culture. Draw the bacterial growth

curve for typical batch culture. [Marks 4 + 6 + 5]

11.a) Give the ways to increase the yield of microbes.

b) What do you mean by bioremediation ? Write a short

note on the degradation of heavy metals.

c) Which microorganisms is used to clear the oilspills ?

Describe the process.

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