University: West Bengal University
Course: B.sc
Subject : Microbial Biotechnology
Year of Question Paper : 2013
CS/B.Sc (H)/Micro. Bio./SEM-4/MBT-404/2013
2013
MICROBIAL BIOTECHNOLOGY
Time Allotted : 3 Hours Full Marks : 70
The figures in the margin indicate full marks.
Candidates are required to give their answers in their own words
as far as practicable.
GROUP – A
( Multiple Choice Type Questions )
1. Choose the correct alternatives for any ten of the
following : [Marks 10 × 1 = 10]
i) The following compound is a secondary metabolite
a) amino acid b) vitamins
c) hormones d) steroids.
ii) The main component of biogas
a) oxygen b) nitrogen
c) carbon dioxide d) methane.
iii) The trickling filter is used in waste water treatment in
a) primary treatment b) secondary treatment
c) tertiary treatment d) preliminary treatment.
iv) The organism which produces parasporal crystal
a) Bacillus subtilis b) Bacillus cereus
c) Pseudomonous sp. d) Bacillus thuringiensis.
v) The organism having OCT plasmid
a) Streptococcus pneumoniae
b) Staphylococcus aureus
c) Bacilus subtilis
d) Pseudomonas putida.
vi) The molecular weight of active Bt toxin is
a) 200 KDa b) 50 KDa
c) 60 KDa d) 100 KDa.
vii) Which of the following expression represents Monod
Equatioin ?
a) µ = µmax b) µ = µmax S / Ks
c) µ = µmaxS / Ks + S d)µ = µmaxS + Ks/S .
viii) The type of bacteria involved in bio-mineralization of ore
is
a) Mesophilic b) Thermophilic
c) Acidophilic d) All of these.
ix) The bioreactor used for pigment synthesis
a) Air lift b) Photo bioreactor
c) Bubble column d) Stirred tank.
x) The catalyst used in biodiesel production
a) Methanol b) Ethanol
c) Sodium hydroxide d) Potassium hydroxide.
xi) The gene of TOL plasmid responsible for production of
xylene oxidase
a) XyIC b) XyIA
c) XyID d) XyIL.
xii) Antifoam used in the fermentor is
a) Mustard oil b) Silicon oil
c) Olive oil d) None of these.
GROUP – B
( Short Answer Type Questions )
Answer any three of the following. [Marks 3 × 5 = 15]
2. Derive the equation for generation time ( g )
from the equation of mean growth rate equation ( k ).
3. Write a short note on air-lift fermentor.
4. Give the method of production of penicillin.
5. Differentiate between primary and secondary metabolites and
also their modes of production.
6. Describe the role of microorganisms in the treatment of
agricultural waste water.
GROUP – C
( Long Answer Type Questions )
Answer any three of the following. [Marks 3 × 15 = 45]
7. Write short notes on the following : [Marks 3 × 5]
a) Bioremediation
b) Biosorption
c) Bioleaching.
8. Define biodiesel. State the chemistry involved in the
production of biodiesel and discuss the role of it as a biofuel
used in transport processes. [Marks 3 + 8 + 4]
9. Describe the process of Orleans method for vinegar
production. What is the role of acidifcation in vinegar
production ? Why acidification if never recommended before
the completion of sugar fermentation ? What is the cause for
darkening of vinegar ? Name the genera for acetic acid
bacteria. Write the reaction for acetic acid production where
oxygen is required. [Marks 5 + 2 + 2 + 2 + 2 + 2]
10. Define chemostat and turbidostat. Compare between batch,
fedbatch and continuous culture. Draw the bacterial growth
curve for typical batch culture. [Marks 4 + 6 + 5]
11.a) Give the ways to increase the yield of microbes.
b) What do you mean by bioremediation ? Write a short
note on the degradation of heavy metals.
c) Which microorganisms is used to clear the oilspills ?
Describe the process.
=============...
Course: B.sc
Subject : Microbial Biotechnology
Year of Question Paper : 2013
CS/B.Sc (H)/Micro. Bio./SEM-4/MBT-404/2013
2013
MICROBIAL BIOTECHNOLOGY
Time Allotted : 3 Hours Full Marks : 70
The figures in the margin indicate full marks.
Candidates are required to give their answers in their own words
as far as practicable.
GROUP – A
( Multiple Choice Type Questions )
1. Choose the correct alternatives for any ten of the
following : [Marks 10 × 1 = 10]
i) The following compound is a secondary metabolite
a) amino acid b) vitamins
c) hormones d) steroids.
ii) The main component of biogas
a) oxygen b) nitrogen
c) carbon dioxide d) methane.
iii) The trickling filter is used in waste water treatment in
a) primary treatment b) secondary treatment
c) tertiary treatment d) preliminary treatment.
iv) The organism which produces parasporal crystal
a) Bacillus subtilis b) Bacillus cereus
c) Pseudomonous sp. d) Bacillus thuringiensis.
v) The organism having OCT plasmid
a) Streptococcus pneumoniae
b) Staphylococcus aureus
c) Bacilus subtilis
d) Pseudomonas putida.
vi) The molecular weight of active Bt toxin is
a) 200 KDa b) 50 KDa
c) 60 KDa d) 100 KDa.
vii) Which of the following expression represents Monod
Equatioin ?
a) µ = µmax b) µ = µmax S / Ks
c) µ = µmaxS / Ks + S d)µ = µmaxS + Ks/S .
viii) The type of bacteria involved in bio-mineralization of ore
is
a) Mesophilic b) Thermophilic
c) Acidophilic d) All of these.
ix) The bioreactor used for pigment synthesis
a) Air lift b) Photo bioreactor
c) Bubble column d) Stirred tank.
x) The catalyst used in biodiesel production
a) Methanol b) Ethanol
c) Sodium hydroxide d) Potassium hydroxide.
xi) The gene of TOL plasmid responsible for production of
xylene oxidase
a) XyIC b) XyIA
c) XyID d) XyIL.
xii) Antifoam used in the fermentor is
a) Mustard oil b) Silicon oil
c) Olive oil d) None of these.
GROUP – B
( Short Answer Type Questions )
Answer any three of the following. [Marks 3 × 5 = 15]
2. Derive the equation for generation time ( g )
from the equation of mean growth rate equation ( k ).
3. Write a short note on air-lift fermentor.
4. Give the method of production of penicillin.
5. Differentiate between primary and secondary metabolites and
also their modes of production.
6. Describe the role of microorganisms in the treatment of
agricultural waste water.
GROUP – C
( Long Answer Type Questions )
Answer any three of the following. [Marks 3 × 15 = 45]
7. Write short notes on the following : [Marks 3 × 5]
a) Bioremediation
b) Biosorption
c) Bioleaching.
8. Define biodiesel. State the chemistry involved in the
production of biodiesel and discuss the role of it as a biofuel
used in transport processes. [Marks 3 + 8 + 4]
9. Describe the process of Orleans method for vinegar
production. What is the role of acidifcation in vinegar
production ? Why acidification if never recommended before
the completion of sugar fermentation ? What is the cause for
darkening of vinegar ? Name the genera for acetic acid
bacteria. Write the reaction for acetic acid production where
oxygen is required. [Marks 5 + 2 + 2 + 2 + 2 + 2]
10. Define chemostat and turbidostat. Compare between batch,
fedbatch and continuous culture. Draw the bacterial growth
curve for typical batch culture. [Marks 4 + 6 + 5]
11.a) Give the ways to increase the yield of microbes.
b) What do you mean by bioremediation ? Write a short
note on the degradation of heavy metals.
c) Which microorganisms is used to clear the oilspills ?
Describe the process.
=============...
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