Solapur University Question Paper
B.Sc. – II (Biotechnology) (Semester – IV) Examination, 2014
MOLECULAR BIOLOGY – II
(Regulation and Gene Expression)
Day and Date : Friday, 25-4-2014 Max. Marks : 50
Time : 11.00 a.m. to 1.00 p.m.
Instructions :1) All the questions are compulsory.
2) Figures to the right indicates full marks.
3) Draw a neat labelled diagrams wherever necessary.
1. Rewrite the following sentences by choosing correct alternatives : 10
1) In eukaryotic transcription, rRNA molecules are synthesized by ___________
a) RNA polymerase – I b) RNA polymerase – II
c) RNA polymerase – III d) RNA polymerase – IV
2) _________________ site on DNA, responsible for binding of enzyme RNA
polymerase during transcription process.
a) Operator b) Promoter c) Regulator d) Enhancer
3) In prokaryotic transcription process, the promoter sequences are recognized
by _________________ subunit of RNA polymerase.
a) Alpha b) Beta c) Omega d) Sigma factor
4) In prokaryotes, the mRNA molecule is _________________
a) Monocistronic b) Polycistronic c) Unicistronic d) Bicistronic
5) In prokaryotic translation process, the initiator tRNA molecule is ___________
a) t-RNAfmet b) t-RNAmet c) t-RNALeu d) t-RNAAla
6) In prokaryotic translation process, the Shinde-Dalgarno sequences are __________
a) AGG AGG U b) GAG GAG U c) UAG UAG U d) GGA GGA U
7) The first step in translation is _________________
a) Transfer of amino acid b) Initiation of synthesis of t-RNA
c) Activation of amino acid d) None of these
8) The operon model is proposed by _________________ in 1961.
a) Jacob and Monod b) H. G. Khurana
c) Robert Holly d) W. M. Nizenberg
SLR-D – 26
9) Lactose operon has _________________ structural genes in sequential manner.
a) Lac-z, Lac-c, Lac-y b) Lac-z, Lac-y, Lac-a
c) Lac-x, Lac-y, Lac-z d) Lac-z, Lac-y, Lac-x
10) In trp operon, tryptophan is acts as _________________
a) Repressor molecule b) Binding protein
c) Co-repressor molecule d) Inducer molecule
2. Answer any five of the following : 10
1) What is meant by splic eosome ?
2) What is operon ?
3) Define polysomes.
4) What is molecular chaperons ?
5) CTD domain of RNA polymerase – II.
6) What is meant by repressor protein ?
3. A) Answer any two of the following : 6
1) Inhibitors of transcription.
2) Ribosome’s.
3) Initiation of translation process in eukaryotes.
B) Write a note on mRNA processing in eukaryotes. 4
4. Answer any two of the following : 10
1) Explain regulation of transcription in prokaryotes.
2) Describe in detail regulation of translation.
3) Describe regulation of operon in Lac operon.
5. Answer any two of the following : 10
1) Explain process of translation in prokaryotes.
2) Post-translational modifications of proteins.
3) Regulation of transcription in eukaryotes with two suitable examples.
_______________
B.Sc. – II (Biotechnology) (Semester – IV) Examination, 2014
MOLECULAR BIOLOGY – II
(Regulation and Gene Expression)
Day and Date : Friday, 25-4-2014 Max. Marks : 50
Time : 11.00 a.m. to 1.00 p.m.
Instructions :1) All the questions are compulsory.
2) Figures to the right indicates full marks.
3) Draw a neat labelled diagrams wherever necessary.
1. Rewrite the following sentences by choosing correct alternatives : 10
1) In eukaryotic transcription, rRNA molecules are synthesized by ___________
a) RNA polymerase – I b) RNA polymerase – II
c) RNA polymerase – III d) RNA polymerase – IV
2) _________________ site on DNA, responsible for binding of enzyme RNA
polymerase during transcription process.
a) Operator b) Promoter c) Regulator d) Enhancer
3) In prokaryotic transcription process, the promoter sequences are recognized
by _________________ subunit of RNA polymerase.
a) Alpha b) Beta c) Omega d) Sigma factor
4) In prokaryotes, the mRNA molecule is _________________
a) Monocistronic b) Polycistronic c) Unicistronic d) Bicistronic
5) In prokaryotic translation process, the initiator tRNA molecule is ___________
a) t-RNAfmet b) t-RNAmet c) t-RNALeu d) t-RNAAla
6) In prokaryotic translation process, the Shinde-Dalgarno sequences are __________
a) AGG AGG U b) GAG GAG U c) UAG UAG U d) GGA GGA U
7) The first step in translation is _________________
a) Transfer of amino acid b) Initiation of synthesis of t-RNA
c) Activation of amino acid d) None of these
8) The operon model is proposed by _________________ in 1961.
a) Jacob and Monod b) H. G. Khurana
c) Robert Holly d) W. M. Nizenberg
SLR-D – 26
9) Lactose operon has _________________ structural genes in sequential manner.
a) Lac-z, Lac-c, Lac-y b) Lac-z, Lac-y, Lac-a
c) Lac-x, Lac-y, Lac-z d) Lac-z, Lac-y, Lac-x
10) In trp operon, tryptophan is acts as _________________
a) Repressor molecule b) Binding protein
c) Co-repressor molecule d) Inducer molecule
2. Answer any five of the following : 10
1) What is meant by splic eosome ?
2) What is operon ?
3) Define polysomes.
4) What is molecular chaperons ?
5) CTD domain of RNA polymerase – II.
6) What is meant by repressor protein ?
3. A) Answer any two of the following : 6
1) Inhibitors of transcription.
2) Ribosome’s.
3) Initiation of translation process in eukaryotes.
B) Write a note on mRNA processing in eukaryotes. 4
4. Answer any two of the following : 10
1) Explain regulation of transcription in prokaryotes.
2) Describe in detail regulation of translation.
3) Describe regulation of operon in Lac operon.
5. Answer any two of the following : 10
1) Explain process of translation in prokaryotes.
2) Post-translational modifications of proteins.
3) Regulation of transcription in eukaryotes with two suitable examples.
_______________
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