University:West Bengal University of Technology
Course: B.Sc
Subject : MOLECULAR GENETICS
Year of Question Paper : 2012
CS/B.Sc.(H)/BT/GENE/MOLBIO/MICROBIO/SEM-4/MOG-401/2012
2012
MOLECULAR GENETICS
Time Allotted : 3 Hours Full Marks : 70
The figures in the margin indicate full marks.
Candidates are required to give their answers in their own words as
far as practicable.
GROUP – A
( Multiple Choice Type Questions )
1. Choose the correct alternatives for any ten of the following :
10 × 1 = 10
i) Type II restriction enzymes have which of the following
characteristics ?
a) Repetitive sequence b) Conserved sequence
c) Palindromic sequence d) None of these.
ii) Which of the following parameters is taken in
consideration during primer designing ?
a) Length of DNA b) GC content
c) Both (a) & (b) d) None of these.
iii) Which one requires NAD+ ?
a) T4 DNA ligase b) E.coli DNA ligase
c) Both of these d) None of these.
iv) What does PUC stand for ?
a) Produced by the University of California
b) Produced by the University of Cambridge
c) Produced by the University of Colombia
d) Produced by the University of Cornell.
v) Cloning requires all the following, excepta) Restriction enzyme b) DNA ligase
c) Methylase d) Vector.
vi) BamH1 is a ................. type of restriction enzyme.
a) Type I b) Type II
c) Type III d) None of these.
vii) In southern blotting experiment, the binding of
transferred DNA to the Nitrocellulose membrane is
............... type.
a) ionic b) covalent
c) hydrophobic d) van der Waals.
viii) If a probe length is less than 200 b.p. ............. is
most useful.
a) Nylon membrane
b) Nitrocellulose membrane
c) Whatman filter paper
d) None of these.
ix) Denaturants such as formamide cause ds DNA to melt
by
a) decreasing the repulsion between phosphates
b) increasing the hydrophobic interactions between
bases
c) increasing the repulsion between the phosphates
d) decreasing the hydrophobic interactions between
bases.
x) The technique of RT-PCR is best suitable for detection
of
a) Allele b) Pathogen
c) Amplicon d) RNA.xi) Which one is a reporter gene ?
a) Lac Z b) Luciferase
c) CAT d) All of these.
xii) Which one is employed in the DNA hybridization
assay ?
a) Etbr
b) Enzyme
c) Chemiluminiscent moiety
d) None of these.
GROUP – B
( Short Answer Type Questions )
Answer any three of the following. 3 × 5 = 15
2. Describe Dot blot hybridization.
3. What is linker and its application ? How is it different from
Adaptors ?
4. Write short notes on the following :
a) M13 Phage vector.
b) Micro-array
c) Nested PCR
d) RAPD.
5. Describe multiplex PCR.
6. What is SDS PAGE electrophoresis ? What is the significance
of using SDS in this technique ?
GROUP – C
( Long Answer Type Questions )
Answer any three of the following. 3 × 15 = 45
7. What is Cloning ? Write down the steps of gene cloning. Why
Alkaline Phosphate treatment is needed in cloning ? Write ashort note on Nick Translation. 2 +
5 + 3 + 5
8. What are the properties of good vector ? What is Blue-White
screening ? What is Agobacterium based plasmid vector ?
Discuss the mechanism of T-DNA transfer. 4 + 4 + 3 + 4
9. Describe Colony Hybridization techniques. What is Protein
blotting Technique ? Briefly describe the technique. What
kind of probe is used in Southern blotting ? 5 + 2 + 6 + 2
10. Write short notes on any three of the following :
a) Maxam and Gilber's chemical degradation method.
b) Gel Electrophoresis.
c) Sanger and Coulson method of DNA sequencing
d) Site Directed mutagenesis.
11. Write short notes on any three of the following : 3 × 5 = 15
a) Nested PCR
b) Insertional inactivation
c) Red white screening
d) Site directed mutagenesis.
========..
Course: B.Sc
Subject : MOLECULAR GENETICS
Year of Question Paper : 2012
CS/B.Sc.(H)/BT/GENE/MOLBIO/MICROBIO/SEM-4/MOG-401/2012
2012
MOLECULAR GENETICS
Time Allotted : 3 Hours Full Marks : 70
The figures in the margin indicate full marks.
Candidates are required to give their answers in their own words as
far as practicable.
GROUP – A
( Multiple Choice Type Questions )
1. Choose the correct alternatives for any ten of the following :
10 × 1 = 10
i) Type II restriction enzymes have which of the following
characteristics ?
a) Repetitive sequence b) Conserved sequence
c) Palindromic sequence d) None of these.
ii) Which of the following parameters is taken in
consideration during primer designing ?
a) Length of DNA b) GC content
c) Both (a) & (b) d) None of these.
iii) Which one requires NAD+ ?
a) T4 DNA ligase b) E.coli DNA ligase
c) Both of these d) None of these.
iv) What does PUC stand for ?
a) Produced by the University of California
b) Produced by the University of Cambridge
c) Produced by the University of Colombia
d) Produced by the University of Cornell.
v) Cloning requires all the following, excepta) Restriction enzyme b) DNA ligase
c) Methylase d) Vector.
vi) BamH1 is a ................. type of restriction enzyme.
a) Type I b) Type II
c) Type III d) None of these.
vii) In southern blotting experiment, the binding of
transferred DNA to the Nitrocellulose membrane is
............... type.
a) ionic b) covalent
c) hydrophobic d) van der Waals.
viii) If a probe length is less than 200 b.p. ............. is
most useful.
a) Nylon membrane
b) Nitrocellulose membrane
c) Whatman filter paper
d) None of these.
ix) Denaturants such as formamide cause ds DNA to melt
by
a) decreasing the repulsion between phosphates
b) increasing the hydrophobic interactions between
bases
c) increasing the repulsion between the phosphates
d) decreasing the hydrophobic interactions between
bases.
x) The technique of RT-PCR is best suitable for detection
of
a) Allele b) Pathogen
c) Amplicon d) RNA.xi) Which one is a reporter gene ?
a) Lac Z b) Luciferase
c) CAT d) All of these.
xii) Which one is employed in the DNA hybridization
assay ?
a) Etbr
b) Enzyme
c) Chemiluminiscent moiety
d) None of these.
GROUP – B
( Short Answer Type Questions )
Answer any three of the following. 3 × 5 = 15
2. Describe Dot blot hybridization.
3. What is linker and its application ? How is it different from
Adaptors ?
4. Write short notes on the following :
a) M13 Phage vector.
b) Micro-array
c) Nested PCR
d) RAPD.
5. Describe multiplex PCR.
6. What is SDS PAGE electrophoresis ? What is the significance
of using SDS in this technique ?
GROUP – C
( Long Answer Type Questions )
Answer any three of the following. 3 × 15 = 45
7. What is Cloning ? Write down the steps of gene cloning. Why
Alkaline Phosphate treatment is needed in cloning ? Write ashort note on Nick Translation. 2 +
5 + 3 + 5
8. What are the properties of good vector ? What is Blue-White
screening ? What is Agobacterium based plasmid vector ?
Discuss the mechanism of T-DNA transfer. 4 + 4 + 3 + 4
9. Describe Colony Hybridization techniques. What is Protein
blotting Technique ? Briefly describe the technique. What
kind of probe is used in Southern blotting ? 5 + 2 + 6 + 2
10. Write short notes on any three of the following :
a) Maxam and Gilber's chemical degradation method.
b) Gel Electrophoresis.
c) Sanger and Coulson method of DNA sequencing
d) Site Directed mutagenesis.
11. Write short notes on any three of the following : 3 × 5 = 15
a) Nested PCR
b) Insertional inactivation
c) Red white screening
d) Site directed mutagenesis.
========..
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