B.Sc. I (Semester – II), Industrial Microbiology Paper – III (New) ENTREPRENEURSHIP,2014 Question Paper,Solapur University Question Paper

Solapur University Question Paper
B.Sc. I (Semester – II) (Entrepreneurship)Examination, 2014
 Industrial Microbiology Paper – III (New)
ENTREPRENEURSHIP
 (Fundamentals of Microbiology)
Day and Date : Thursday, 15-5-2014 Max. Marks : 50
Time : 11.00 a.m. to 10.00 p.m.
N.B. : 1) All questions are compulsory.
2) Draw a neat labelled diagram wherever necessary.
3) Figures to the right indicates full marks.
1. Rewrite the following sentences by selecting correct alternatives given below : 10
i) Indicator plate technique is used for primary screening of _________
producing organisms
a) Antibiotic b) Amylase
c) Organic acids and amines d) vitamin
ii) Major component of molasses is
a) Sugar b) Protein
c) Vitamin d) Lipid
iii) The raw material having __________ is rejected for preparation of fermentation
media.
a) Less C and N sources b) Less foaming
c) Buffering capacity d) More C and N sources
iv) From ____________ of fermentor samples are removed and analysed time to
time.
a) Retension tube b) Bottom drain
c) Sampling point d) Head space
Seat
No.
SLR-EN – 51 -2-
v) ___________ is used as precursor for vitamin B12 production.
a) Chloride b) Cyanide
c) Phenyl acetic acid d) Antranilic acid
vi) Microbial inhibition spectrum of antibiotic is determined by __________
technique.
a) Standard plate count b) Crowded plate
c) Replica plate d) Giant colony
vii) ______________ material for construction of fermentor in lactic acid and beer
production is rejected.
a) Copper b) Wooden
c) Iron d) Stainless steel
viii) In fermentation process agitation of the medium is done by using
a) Spargers b) Baffles
c) Impellars d) Bottom drain
ix) _____________ is used as growth factor in medium used for glutamic acid
production.
a) Corn steep liquor b) Suitite waste liquor
c) Biotin d) Calcium pantothenate
x) _____________ is crude organic antifoam agent.
a) Silicon compound b) Vegifat – X
c) Lard oil d) Phenyl acetic acid
2. Answer any five of the following : 10
i) Write applications of fermentor.
ii) Give the examples of antifoam agents.
iii) What is the meaning of serial subculture ?
iv) Define the screening.
v) Define the industrial microbiology.
vi) Write the significance of the buffers in fermentation process.
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3. A) Answer the following questions in brief (any two) : 6
i) Write the scope of industrial microbiology.
ii) Describe the primary screening.
iii) Describe the preservation of stock cultures by Lyophilization.
B) Describe in brief sterilization of fermentation media and fermentor. 4
4. Write short notes on any two : 10
i) Typical fermentor with diagram.
ii) Secondary screening.
iii) Factors affecting on fermentation process.
5. Describe in detail any one of the following : 10
i) Fermentation media
ii) Preservation of stock cultures.
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