Solapur University Question Paper
B.Sc. (Part – III) (Semester – V) Examination, 2014
BIOTECHNOLOGY
Fermentation Technology
Day and Date : Friday, 11-4-2014 Max. Marks : 50
Time : 3.00 p.m. to 5.00 p.m.
N.B. : 1) All questions are compulsory.
2) Figures to the right indicate full marks.
1. Choose the correct alternative and rewrite the sentence again. 10
1) _________________ is the crude organic antifoam agent.
a) Silicon compound b) Phenyl acetic acid
c) Lard oil d) Citric acid
2) Indicator plate technique is used for screening of organisms producing
_________________
a) Organic acids and amines b) Vitamins
c) Antibiotics d) Gases
3) For turbidimetric assays _________________ media are used.
a) Solid b) Semisolid
c) Liquid d) Solid and liquid
4) Economic fermentation of an industrial product is done by using
_________________
a) Semi synthetic media b) Living media
c) Synthetic media d) Waste as raw material
5) In ethanol production, the concentration of molasses is adjusted between
_________________
a) 10 – 18% b) 50 – 55% c) 30 – 40% d) 1 – 10%
SLR-D – 33 -2-
6) _________________ is the water extract by-product from the dairy industry.
a) Cornsteep liquor b) Whey
c) Sulfite waste liquor d) Molasses
7) Solvent extraction is used for recovery of _________________
a) alcohol b) amylase
c) penicillin d) none of these
8) Scale up study is started with _________________
a) Erlenmeyers flasks b) Pilot plants
c) Seed tank d) Production tank
9) _________________ technique is used for primary screening of antibiotic
producers.
a) Giant colony b) Crowded plate
c) Indicator plate d) Streak plate
10) The submerged culture production of amylase is carried out by
_________________
a) Aspergillus niger b) E. coli
c) Saccharomyces cerevisiae d) B. subtilis
2. Attempt any five of the following : 10
1) Enlist the microorganisms used for penicillin production.
2) Write down the applications of citric acid.
3) Define – Bioinsecticide.
4) Which are the different microbiological assays ?
5) Enlist the methods of culture preservation.
6) Define – Screening.
-3- SLR-D – 33
3. A) Write short answers on (any 2) : 6
1) Give an account of raw materials used in fermentation.
2) Recovery of vitamin B12.
3) Production strain in fermentation.
B) Give an account of basic design of fermentor. 4
4. Write short notes on (any 2) : 10
1) Scale up of fermentation.
2) Citric acid fermentation.
3) Precipitation and Distillation as recovery process.
5. Write short notes on (any 2) : 10
1) Use of computer in fermentation technology.
2) Sterilization of media.
3) Strain improvement.
_____________________
B.Sc. (Part – III) (Semester – V) Examination, 2014
BIOTECHNOLOGY
Fermentation Technology
Day and Date : Friday, 11-4-2014 Max. Marks : 50
Time : 3.00 p.m. to 5.00 p.m.
N.B. : 1) All questions are compulsory.
2) Figures to the right indicate full marks.
1. Choose the correct alternative and rewrite the sentence again. 10
1) _________________ is the crude organic antifoam agent.
a) Silicon compound b) Phenyl acetic acid
c) Lard oil d) Citric acid
2) Indicator plate technique is used for screening of organisms producing
_________________
a) Organic acids and amines b) Vitamins
c) Antibiotics d) Gases
3) For turbidimetric assays _________________ media are used.
a) Solid b) Semisolid
c) Liquid d) Solid and liquid
4) Economic fermentation of an industrial product is done by using
_________________
a) Semi synthetic media b) Living media
c) Synthetic media d) Waste as raw material
5) In ethanol production, the concentration of molasses is adjusted between
_________________
a) 10 – 18% b) 50 – 55% c) 30 – 40% d) 1 – 10%
SLR-D – 33 -2-
6) _________________ is the water extract by-product from the dairy industry.
a) Cornsteep liquor b) Whey
c) Sulfite waste liquor d) Molasses
7) Solvent extraction is used for recovery of _________________
a) alcohol b) amylase
c) penicillin d) none of these
8) Scale up study is started with _________________
a) Erlenmeyers flasks b) Pilot plants
c) Seed tank d) Production tank
9) _________________ technique is used for primary screening of antibiotic
producers.
a) Giant colony b) Crowded plate
c) Indicator plate d) Streak plate
10) The submerged culture production of amylase is carried out by
_________________
a) Aspergillus niger b) E. coli
c) Saccharomyces cerevisiae d) B. subtilis
2. Attempt any five of the following : 10
1) Enlist the microorganisms used for penicillin production.
2) Write down the applications of citric acid.
3) Define – Bioinsecticide.
4) Which are the different microbiological assays ?
5) Enlist the methods of culture preservation.
6) Define – Screening.
-3- SLR-D – 33
3. A) Write short answers on (any 2) : 6
1) Give an account of raw materials used in fermentation.
2) Recovery of vitamin B12.
3) Production strain in fermentation.
B) Give an account of basic design of fermentor. 4
4. Write short notes on (any 2) : 10
1) Scale up of fermentation.
2) Citric acid fermentation.
3) Precipitation and Distillation as recovery process.
5. Write short notes on (any 2) : 10
1) Use of computer in fermentation technology.
2) Sterilization of media.
3) Strain improvement.
_____________________
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