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Tuesday, May 23, 2017

MS University GAMI41 Fundamentals of Immunology April 2016 Question Paper

Manonmaniam Sundaranar University Question Paper
CODE: 30975 SUB CODE: GAMI41
BSC (CBCS) DEGREE EXAMINATION, APRIL 2016
FOURTH SEMESTER
MICROBIOLOGY – ALLIED
FUNDAMENTALS OF IMMUNOLOGY

PART A ( 10 * 1 = 10 MARKS )
CHOOSE THE CORRECT ANSWER:

1. Replication of DNA takes place in
(a) Conservation manner
(b) Dispersive manner
(c) Semi-conservative manner
(d) Takes place only in the 3' to 5'

2. Which of the followinng will not be found in DNA?
(a) Thymine
(b) Uracil
(c) Adenine
(d) Guanine

3. A nucleotide is composed of
(a) An acid, a base, and a sugar
(b) A base, a sugar and a phosphate
(c) A base, and sugar
(d) An acid, a sugar, and a phosphate

4. Oligonucleotide are formed from 2 to 10
(a) Strands
(b) Hydrogen bonds
(c) Nucleosides
(d) Nucleotides

5. The variable portion of DNA is the sequence of _____________
(a) Phosphoric acids
(b) Sugars
(c) Bases
(d) Phosphates

6. Which pair is a complementary base pair?
(a) C-G
(b) A-G
(c) A-C
(d) G-C

7. Which is the following is not associated with genetic engineering?
(a) Translation
(b) Transformation
(c) Cloning
(d) Expression

8. Which of the following is not a restriction endonuclease?
(a) Eco RI
(b) DNA ligase
(c) Hind III
(d) Bam H1

9. DNA sequencing is done by
(a) Maxam Gilbert methods
(b) Sanger dideoxy method
(c) Both (a) and (b)
(d) Watson and crick

10. The Polymerase chain reaction is a techniques used for?
(a) Used to demonstrate DNA as the genetic material.
(b) Used to determine the content of minerals in a soil sample.
(c) Uses short DNA primers and a thermostable DNA polymerase to replicate specific DNA sequences in vitro.
(d) Measures the ribosome transfer rate during translation.

PART B – ( 5 * 5 = 25 MARKS )
ANSWER ALL QUESTION. CHOOSING EITHER (a) OR (B)

11. (a) Write short note on central dogma.
Or
(b) Explain the double standard DNA.

12. (a) Explain about the role of the restriction enzyme in genetic engineering.
Or
(b) Write a short note on pBR322.

13. (a) Write short note on plasmid and cosmids.
Or
(b) Clarification on transposons.

14. (a) Write a short note on transcription.
Or
(b) Explain southern blotting.

15. (a) Write about uses of E.COLI in the genetic engineering.
Or
(b) Explain in detailed about steps employed in PCR.

PART C – ( 5 * 8 = 40 MARKS )
ANSWER ALL QUESTION. CHOOSING EITHER (a) OR (B).

16. (a) Explain in detail any four tools esed in genetic engineering.
Or
(b) Essay on protein synthesis.
.
17. (a) Explain about the restriction enzymes and its application.
Or
(b) Essay on nomenclature of restriction enzymes and explain the Hind III and EcoRI.

18. (a) Elaborate essay on cosmids and its application.
Or
(b) Explain the artificial vectors and transposons.

19. (a) Elaborate essay on prokaryotic gene cloning with suitable diagram.
Or
(b) Explain in detail about southern blotting and western blotting.

20. (a) Write about the pricple of RT-PCR and its application.
Or
(b) Write about Griffith experiments.

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