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Tuesday, December 29, 2015

Second Year B. Pharmacy,2010 Question Paper,University Of Pune Question Paper,2.2 : PHARMACEUTICAL MICROBIOLOGY,

University Of Pune Question Paper
Second Year B. Pharmacy Examination, 2010
2.2 : PHARMACEUTICAL MICROBIOLOGY
(2004 Course)
Time : 3 Hours Max. Marks : 80
 Instructions:1) Q. No.s 1 and 5 are compulsory. Out of the remaining,
attempt 2 questions from Section 1 and 2 questions from
Section II.
2) Answers to the two Sections should be written in separate
books.
3) Neat diagrams must be drawn wherever necessary.
4) Black figures to the right indicate full marks.
SECTION – I
1. Answer the following : 10
a) State the reason for using cedar wood oil under 100X objective.
b) Define :
i) exotoxin ii) selective media
c) State the correlation between capsule and virulence of bacteria
d) Enlist the media used in isolation of Staphylococcus aureus with reasons.
e) Give the importance of fungi.
2. a) Describe the compound microscope. 7
b) Explain in detail one of the method of multiplication of a bacteriophage. 8
3. Answer the following : 15
a) State the methods of enumeration of bacteria and describe any one method.
b) Diagrammatically describe the gram positive cell wall with example.
c) State the methods of preservation of microbial cultures.
4. Write short notes on (any three) : 15
a) Salmonella
b) Preservation of pharmaceutical products
c) Conjugation
d) Sporulation.
SECTION – II
5. Answer the following : 10
a) Which tests are done for microbial limit test of starch ?
b) What are interferons ? Give their significance.
c) What is the action of cholera toxin ?
d) What is the importance of Aspergillus ?
e) Write action of ultra violet light on bacteria and their use in sterilization.
6. a) Describe in brief general method of vaccine production. 8
b) What is complement ? Describe classical complement pathway. 7
7. a) Describe different classes, action and uses of disinfectants. 8
b) Write on sterility test of pharmaceutical products. 7
8. Write short note on (any 3) : 15
a) Radiation sterilization
b) Vitamin B12 assay
c) Normal flora of human body
d) Kelsey – Syke’s test.
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